Journal: PLoS ONE
Article Title: Maternal Bisphenol A Exposure Impacts the Fetal Heart Transcriptome
doi: 10.1371/journal.pone.0089096
Figure Lengend Snippet: Whole transcriptome analysis was performed on the left ventricle (LV), right ventricle (RV), left atrium (LA) and the right atrium (RA) of rhesus monkey (Macaca mulatta) fetuses that were exposed maternally to a 400 µg/kg body weight, Bisphenol A (BPA), relative to matched control fetuses, either during, (A) early gestation, EG (days; 50–100±2) or, (B) late gestation, LG (days 100±2–term). Genes that changed by greater than ±1 log 2 fold change (1 LFC = 2 fold change) at an unadjusted p≤0.01 were considered significant and differentially expressed. Bars represent the number of upregulated (positive y-axis) or downregulated (negative y-axis) genes in each of the tissues.
Article Snippet: Due to the limitations in the quantity of tissue available from the LA and RA, cDNA preparations from both these tissue were prepared by employing a PCR based amplification approach available through TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2) (Sigma, St. Louis, MO, USA).
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